1주차 |
Introduction and team grouping |
2주차 |
Isolation of chromosomal DNA from bacteria |
3주차 |
Polymerase chain reaction (PCR) |
4주차 |
Preparation of competent cells |
5주차 |
Agarose gel electrophoresis of PCR products |
6주차 |
Gel extraction from agarose to isolate the PCR products |
7주차 |
Ligation between PCR products and TA cloning vector |
8주차 |
Transformation of ligation product into competent cell |
9주차 |
Preparation of plasmid vector DNA from transformants |
10주차 |
Agarose gel electrophoresis of plasmid vector DNA treated with restriction enzymes |
11주차 |
Precipitation of DNA by ethanol |
12주차 |
Transformation of recombinant DNA molecule into competent cell |
13주차 |
Gene expression |
14주차 |
Determination of expression product by SDS-PAGE |
15주차 |
Final term examination |
16주차 |
|